Volume 62, Issue 2 (12 2004)                   Tehran Univ Med J 2004, 62(2): 89-98 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Noorizadeh M, Hajati J, Hoseinali Eazad M, Moosavi Shabestari T. Production And Partial Purification Of IL-2 Cells From Jurcat Cell Line Culture. Tehran Univ Med J. 2004; 62 (2) :89-98
URL: http://tumj.tums.ac.ir/article-1-1125-en.html
Abstract:   (5487 Views)

Background: Role of cytokines in regulation of immune system has been the subject of studies and clinical investigations. One of these cytokines, IL-2 has been well initially introduced as T cell Growth factor (TCGF), but subsequently it appeared that IL-2 is one of the important mediators affecting growth, development and activity of T, B, NK and LAK cells. Nowadays this cytokines has extensive use in clinical and research fields of immunotherapy of cancer and infectious disease.

Materials and Methods: In this study, we used Jurcat cell line for production and partial purification of IL-2 106 cell/ml were stimulated by PHA (1 µg/ml) and PMA (10 µg/ml) at the third day of the culture and then supernatant were collected after 22 hrs.

 Results & Conclusion: In order to obtain sufficient amount of IL-2 and eliminate interfacing materials, supernatants were concentrated using Amicon 10 and 30 PM filters. After concentrating, bioassay and Elisa were performed to detect the biological activity and amount of produced IL-2. Reversed phase-HPLC was used to confirm the IL-2 identity and purification.

Full-Text [PDF 1016 kb]   (1494 Downloads)    

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2019 All Rights Reserved | Tehran University Medical Journal TUMS Publications

Designed & Developed by : Yektaweb