Volume 59, Issue 6 (11-2001)                   Tehran Univ Med J 2001, 59(6): 92-97 | Back to browse issues page

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Falahat Pisheh H, Gharavi Noori A. Application of hight performance liquid chromatography with spectrofluorimetric detection for determination of human milk retinol . Tehran Univ Med J. 2001; 59 (6) :92-97
URL: http://tumj.tums.ac.ir/article-1-5631-en.html
Abstract:   (2497 Views)
World health organization has introduced concentration of vitamin A in breast milk as a proper indicator for characterization of vitamin A deficiency in lactating mothers and their infants (<6 mo). A normal phase high performance liquid chromatography with spectrofluorometric detection as a specific and sensitive detector was used for characterization of all-trans retional from 13-Cis retinal. The method was sensitive (0.009 ppm) and accurate (1±0.054 µ mol/l) and had a good recovery percentage (99.36%). This method was more better than before methods and was compatible with the other useful methods. Effect of freezing and defreezing on all-trans retinol content of milk sample was investigated. The result was interesting, whatever all-trans retinol content was bigger, it’s destroying was bigger too. Because of that reseon, day today reproducibility wasn’t good. Standard of retinol was 95% all-trans and pure so we should use this method because two retinol isomers wasn’t separated from each other by reversed phase chromatography and UV detection.
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