Volume 72, Issue 8 (November 2014)                   Tehran Univ Med J 2014, 72(8): 508-514 | Back to browse issues page

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Mostafavi Dehraisi S S, Mehdi Sadat S, Davari Tanha F, Aghasadeghi M R A, Safarpour M, Abbasi Ranjbar P et al . Association of Glutathione S-transferase P1 gene polymorphism (Ile105Val) with uterine leiomyoma in Iranian population. Tehran Univ Med J 2014; 72 (8) :508-514
URL: http://tumj.tums.ac.ir/article-1-6340-en.html
1- Department of Genetic, Islamic Azad University, Ahar Branch, Ahar, Iran.
2- Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
3- Department of Obstetrics, Gynecologist, and Reproductive Endocrinology, Valiasr Reproductive Health Center, Tehran, University of Medical Science, Tehran, Iran.
4- Cellular and Molecular Research Center, Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5- Department of Cellular and Molecular Biology, University of Guilan, Rasht, Iran.
6- Cellular and Molecular Research Center, Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , ae35m@yahoo.com
Abstract:   (6124 Views)
Background: Uterine leiomyoma is one of the most common benign smooth muscle tumors occurring in 20-40% of women worldwide in their reproductive years. Recent studies revealed that estrogen plays an important role in the pathogenesis of this disease. Since glutathione S-transferase (GST) gene family are involved in the biosynthesis of estrogen, the prior probability that variants at this locus are associated with uterine leiomyoma is likely to be above the null. Therefore, this study was carried out to examine whether GSTP1 polymorphism (Ile105Val) is associated with increased risk of uterine leiomyoma in Iranian population. Methods: In this case-control study, 50 women diagnosed with uterine leiomyoma and 50 healthy controls were recruited from subjects referred to the Pasteur Institute of Iran from November 2012 to September 2013. The genomic DNA was extracted from peripheral blood leucocytes using the standard phenol-chloroform method and subsequently the GSTP1 polymorphism was genotyped using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Logistic regression analysis was applied to estimate odds ratios and 95% confidence intervals after age adjustment using the SPSS statistical software package, version 18.0. Results: The results showed significant differences between case and control groups in terms of genotype frequency (P<0.0001). In addition, the results indicated that the presence of the valine allele significantly increased risk of uterine leiomyoma about three times more in individuals carrying the mutant allele compared to control group (Odds Ratio: 3.34 95%CI: 1.82-6.15 P<0.0001). Conclusion: To our knowledge, this is the first study performed in Iranian population assessing the association between GSTP1 Ile105Val polymorphism and risk of uterine leiomyoma. However, further extensive studies with a large number of samples from different populations and ethnicities are required to validate the results obtained in this study.
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