Tehran University Medical Journal
مجله دانشکده پزشکی، دانشگاه علوم پزشکی تهران
Tehran Univ Med J
Medical Sciences
http://tumj.tums.ac.ir
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1683-1764
1735-7322
10.18869/acadpub.tumj
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jalali
1391
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gregorian
2012
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ارزیابی کیفیت، بلوغ هسته و آسیب DNA هسته اسپرم در موشهای سوری بالغ درمان شده با سولپراید
Evaluation of sperm quality, maturation and DNA integrity in adult mice treated with sulpiride
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<!--stripped--></p><strong><span style="font-size: 10pt">زمینه
و هدف</span></strong><span style="font-size: 10pt">:</span><span style="font-size: 10pt"></span><em><span style="font-size: 10pt"> </span></em><span style="font-size: 10pt">مصرف بعضی از داروهای آنتیسایکوتیک
تاثیر شدیدی بر باروری جنس نر دارد. اثرات هیپوفیزی و تغییر در غلظت سرمی بعضی از هورمونها
مثل پرولاکتین، </span><span style="font-size: 7pt">LH</span><span style="font-size: 10pt"> <span lang="AR-SA">و </span></span><span style="font-size: 7pt">FSH</span><span style="font-size: 10pt"> <span lang="AR-SA">تاثیرات مختلفی بر تولید اسپرم
دارد. در این مطالعه اثر داروی سولپراید بر روی کیفیت، میزان آسیب </span></span><span style="font-size: 7pt">DNA</span><span style="font-size: 10pt"> <span lang="AR-SA">و وضعیت بلوغ
هسته اسپرم مطالعه شد. </span></span><strong><em></em></strong>
<p class="MsoNormal" style="direction: rtl unicode-bidi: embed text-align: right"><strong><span style="font-size: 10pt">روش بررسی:</span><em><span style="font-size: 10pt"> </span></em></strong><span style="font-size: 10pt">24 موش نر سوری بالغ (سن:
8-6 هفته) به سه گروه تیمار، کنترل شم و کنترل تقسیم شدند. بهمدت 45 روز روزانه به
گروه تیمار محلول سولپراید بهمیزان </span><span style="font-size: 8pt">mg/kg</span><span style="font-size: 10pt">40 و به کنترل شم حلاّل دارو بهصورت داخل صفاقی تزریق شد. گروه
کنترل چیزی دریافت نکرد. پس از این مدت تمامی موشها به روش جابهجایی گردن کشته شده،
اپیدیدیم به روش جراحی خارج شد و در</span><span style="font-size: 8pt">ml</span><span style="font-size: 10pt"> </span><span style="font-size: 10pt">1 محیط کشت </span><span style="font-size: 8pt">H</span><span style="font-size: 9pt">uman </span><span style="font-size: 8pt">T</span><span style="font-size: 9pt">ubal </span><span style="font-size: 8pt">F</span><span style="font-size: 9pt">luid </span><span style="font-size: 6pt">(</span><span style="font-size: 7pt">HTF)</span><span style="font-size: 11pt"> </span><span style="font-size: 10pt">بهمدت نیم ساعت در داخل
انکوباتور</span><span style="font-size: 7pt">CO2</span><span style="font-size: 10pt"> </span><span style="font-size: 10pt"><span> </span><span lang="AR-SA">به منظور شناورسازی اسپرمها گذاشته
شد. تعداد، قدرت تحرک و قدرت زیستپذیری اسپرمها مورد بررسی قرار گرفت. همچنین کیفیت
بلوغ هسته و میزان آسیب </span></span><span style="font-size: 7pt">DNA</span><span style="font-size: 10pt"> <span lang="AR-SA">اسپرم توسط رنگآمیزی آنیلین بلو و آکریدین
اورنج ارزیابی<span style="color: red"> </span>شد<span style="color: red">.</span></span></span><strong><em></em></strong></p>
<p class="MsoNormal" style="direction: rtl unicode-bidi: embed text-align: right"><strong><span style="font-size: 10pt">یافتهها:</span></strong><span style="font-size: 10pt"> </span><span style="font-size: 10pt">نتایج نشان داد در موشهای
گروه درمانی، کاهش چشمگیری در تعداد و قدرت تحرک اسپرمها مشاهده شد و تعداد اسپرمهای
غیرطبیعی در مقایسه با دو گروه دیگر افزایش معنیداری داشت (05/0</span><span style="font-size: 7pt">P<</span><span style="font-size: 10pt">)</span><span style="font-size: 10pt">. کاهش مشخصی در قدرت زیستپذیری
و بلوغ هسته اسپرمها دیده شد و میزان آسیب </span><span style="font-size: 7pt">DNA</span><span style="font-size: 10pt"> <span lang="AR-SA">در مقایسه با گروههای دیگر
افزایش چشمگیری یافت (05/0</span></span><span style="font-size: 7pt">P<</span><span style="font-size: 10pt">)</span><span style="font-size: 10pt">.</span><span lang="AR-SA" style="font-size: 11pt"> </span></p>
<p style="text-align: right"><strong><span style="font-size: 10pt">نتیجهگیری: </span></strong><span style="font-size: 10pt">مشخص شده داروی آنتیسایکوتیک سولپراید دارای اثرات منفی
بر پارامترهای اسپرم در موشهای درمان شده است و در تعدادی از نمونهها هیپوگنادیسم
ثانویه ایجاد میکند. </span></p>
<p style="text-align: justify"><strong>Background</strong>: Use of certain antipsychotic drugs has severe effects on fertility in males. Hypothalamus and hypophysial impressions and changes in plasma hormones concentration like prolactin, LH and FSH can affect sperm production. In this study, we investigated the effects of sulpiride on sperm quality, maturation and DNA damage.<br><strong></strong></p>
<p style="text-align: justify"><strong>Methods</strong>: Twenty for adult male mice (age: 6-8 weeks) were divided into three groups. The treatment group received 40 mg/kg sulpiride solution and the control sham group was given carrier of the drug intraperitoneally (IP) daily for 45 days but the control group received nothing. Finally, all the mice were sacrificed by cervical dislocation and their cauda epididymis were removed surgically. The excised specimens were placed in 1 ml HTF medium and incubated for 30 min in CO2 incubator to allow the spermatozoa to swim out. Later, sperm count, motility and viability were analyzed. Additionally, sperm chromatin quality and DNA integrity were assessed by aniline blue and acridine orange staining.<br><strong></strong></p>
<p style="text-align: justify"><strong>Results</strong>: Significant decrease in sperm motility and count were observed in the treatment group while the number of abnormal sperm increased as compared with the other two groups. Sperm viability and DNA maturation showed significant reduction and the rate of DNA damage increased in comparison with the control sham and the control groups (P<0.05).<br><strong></strong></p>
<p style="text-align: justify"><strong>Conclusion</strong>: The study showed that sulpiride has negative effects on sperm parameters in treated animals and in some cases it could cause secondary infertility.</p>
سولپراید,کیفیت اسپرم,آسیب DNA,بلوغ هسته
DNA damage,nuclear maturation,sperm quality,sulpiride
205
211
http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-120&slc_lang=fa&sid=1
Ahmadi
A
آرام
احمدی
aram.ahmadi82@gmail.com