Volume 62, Issue 1 (12 2004)                   Tehran Univ Med J 2004, 62(1): 1-9 | Back to browse issues page

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Mohyeddin Bonab M, Moghaddam K A, Alijanipoor P, Beshtar M, Ghavam Zadeh A. The Effect Of 8 Day Storage At 4˚C On Total Nucleated Cell Count, Cell Viability, And Granulocyte-Macrophage Colony Forming Unit Of Mobilized Peripheral Blood. Tehran Univ Med J. 2004; 62 (1) :1-9
URL: http://tumj.tums.ac.ir/article-1-1136-en.html
Abstract:   (4884 Views)

Background: Hematopoietic stem cell (HSC) transplantation has brought the possibility of the use of high dose chemotherapy in the treatment of malignant hematopoietic diseases. Short-term HSC preservation at 4˚C is the most common method for autologous peripheral blood stem cell transplantation (PBSCT).

Materials and Methods: Thirty-seven mobilized PBSC samples from thirteen hematological patients (4 AML, 4 MM and 5 Lymphoma cases) who were selected for autologous PBSCT and 24 normal candidates for allogenic PBSCT were preserved in five separate sterile 2 ml tubes in 4˚C. Each sample was evaluated for total nucleated cell (TNC) count, dye exclusion cell viability and Granulocyte-Macrophage colony forming unit (GM-CFU in semisolid medium after 16 days) in days 0, 2, 4, 6 and 8. The results were converted to percentages of day 0 measures. The data were analyzed by SPSS 10.0 using Paired Samples T test, Independent Samples T test and Regression.

Results: The mean percentages (and standard deviations) of TNC count, cell viability and GM-CFU for days 0, 2, 4, 6 and 8 are shown below: No significant correlation was found between age, sex, weight and the kind of donor with TNC, viability and GM-CFU.

Conclusion: In this study, we have found that during storage of mobilized PBSC in 4˚C, TNC count and cell viability still remains higher than 70% after eight days, while GM-CFU decreases more rapidly and falls to less than 50% after day 4.Therefore, TNC count and cell viability do not decrease as fast as GM-CFU.

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