Volume 72, Issue 6 (September 2014)
Tehran Univ Med J 2014, 72(6): 367-378 |
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Fatemi M J, Khajerahimi A A, Nikoumaram B, Sakhaei M, Mostafavi S, Atashi A, et al . Amniotic membrane seeded with mesenchymal adipose-derived stem cell for coverage of wound in third degree burn: An experimental study. Tehran Univ Med J 2014; 72 (6) :367-378
URL: http://tumj.tums.ac.ir/article-1-6252-en.html
URL: http://tumj.tums.ac.ir/article-1-6252-en.html
Mohammad Javad Fatemi1 
, Amir Asadollah Khajerahimi2 , Babak Nikoumaram2 , Maryam Sakhaei3 , Shabnam Mostafavi3 , Amir Atashi * 4, Mirsepehr Pedram5 , Seyed Hamid Reza Aghayan6 , Shirin Araghi7 , Majid Soltani3 , Majid Mossahebi Mohammadi8
, Amir Asadollah Khajerahimi2 , Babak Nikoumaram2 , Maryam Sakhaei3 , Shabnam Mostafavi3 , Amir Atashi * 4, Mirsepehr Pedram5 , Seyed Hamid Reza Aghayan6 , Shirin Araghi7 , Majid Soltani3 , Majid Mossahebi Mohammadi8
1- Department of Plastic & Recon-structive Surgery, Burn Research Center, Iran University of Medi-cal Sciences, Tehran, Iran.
2- Department of Plastic & Recon-structive Surgery, Islamic Azad University, Tehran Medical Sci-ences Branch, Tehran, Iran.
3- General Practitioner, Burn Re-search Center, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Hematology, Fac-ulty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , atashia@modares.ac.ir
5- Department of Surgery and Ra-diology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
6- Chronic Diseases Research Cen-ter, Endocrinology and Metabo-lism Research Institute, Tehran University of Medical Sciences & SinaCell knowledge Based com-pany, Tehran, Iran.
7- Department of Nursing, Hazrat Fatima Hospital, Burn Research Center, Tehran, Iran.
8- Department of Stem cell Biology, Stem cell Technology Research center, Tehran, Iran.
2- Department of Plastic & Recon-structive Surgery, Islamic Azad University, Tehran Medical Sci-ences Branch, Tehran, Iran.
3- General Practitioner, Burn Re-search Center, Iran University of Medical Sciences, Tehran, Iran.
4- Department of Hematology, Fac-ulty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , atashia@modares.ac.ir
5- Department of Surgery and Ra-diology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
6- Chronic Diseases Research Cen-ter, Endocrinology and Metabo-lism Research Institute, Tehran University of Medical Sciences & SinaCell knowledge Based com-pany, Tehran, Iran.
7- Department of Nursing, Hazrat Fatima Hospital, Burn Research Center, Tehran, Iran.
8- Department of Stem cell Biology, Stem cell Technology Research center, Tehran, Iran.
Abstract: (7999 Views)
Background: Stem cells are applied in the treatment of wide range of diseases and can be separated from different tissues of the body. These cells can treat diseases by cytokine and growth factor secretion and also cell differentiation. Burn wound is a challenging problem of reconstructive surgery and stem cells may help wound healing process. We designed this study to evaluate the beneficial effect of fat derived stem cells for coverage of 3rd degree burn wound.
Methods: This study was experimental and has been done in Burn Research Center of Iran University of Medical Sciences during January 2012 to April 2013. Thirty rats randomly divided to three equal groups. Inguinal fat of 10 rats (one group) were used for preparation of autologous adipose-derived mesenchymal stem cells. Acellular amnion was used as a scaffold for stem cell transfer. Each of the thirty rats had been exposed to a cm deep 3rd degree burn on back area. 24 hours after surgery, the wound was excised and it had been covered by three methods: conventional dressing in the first group, acellular amnion in the second group and acellular amnion seeded with adipose-derived stem cell in the third group. The rate of wound healing and pathologic characteristics was compared in all three groups.
Results: Healing rate and decrease in wounds size was significantly better in acellular amnion seeded with adipose-derived stem cells compared with other two groups at 3rd and 15th days after surgery P<0.01.
Also in histopathology examination, fibroplasia and neovascularization of wounds were significantly better in stem cells group than the other two groups P<0.001.
Conclusion: Acellular amnion seeded with adipose-derived stem cell can result in faster wound healing and better histopathology characteristic. The amnion as a scaffold and the fat derived stem cells as healing accelerator are recommended for coverage of the 3rd degree burn wounds after excision and it may reduce the need for skin graft.
Type of Study: Original Article |
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