دوره 59، شماره 6 - ( 8-1380 )
جلد 59 شماره 6 صفحات 10-1 |
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Poorpak Z, Gazorani P, Ahmadiani A, Kazem Zadeh A. A simple, low cost and fast improved fluorimetric method for Histamine measurement. Tehran Univ Med J 2001; 59 (6) :1-10
URL: http://tumj.tums.ac.ir/article-1-5632-fa.html
URL: http://tumj.tums.ac.ir/article-1-5632-fa.html
بهینه سازی روش فلوریمتری. مجله دانشکده پزشکی، دانشگاه علوم پزشکی تهران. 1380; 59 (6) :1-10
چکیده: (3538 مشاهده)
The well-known fluorimetric method for histamine measurement which is one of the common methods in diagnostic laboratories was modified to accelerate and facilitate measurement of serum histamine levels and decrease the costs and restrictions. The modified method needs only 1 ml of whole blood (or serum) instead of about 10 ml in original method which is difficult almost or impossible specially for children. In addition, very small amounts of the expensive materials are needed and the samples are saved for about 15 days in -20°C which makes no significant changes. Because in most cases, sample can not be read at sampling day, the saving possibility is an advantage for improved method.
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