Volume 82, Issue 11 (February 2025)
Tehran Univ Med J 2025, 82(11): 871-875 |
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Ajdari F, Gheshlaghi A, Shakiba A, Haghighat S, Jamalidoust M, Sarvari J. Frequency of cytomegalovirus in patients with chronic lymphocytic leukemia: a brief report. Tehran Univ Med J 2025; 82 (11) :871-875
URL: http://tumj.tums.ac.ir/article-1-13410-en.html
URL: http://tumj.tums.ac.ir/article-1-13410-en.html
Farkhondeh Ajdari *1 
, Abolfazl Gheshlaghi1 , Aida Shakiba1 , Shirin Haghighat2 , Marzieh Jamalidoust3 , Jamal Sarvari4
, Abolfazl Gheshlaghi1 , Aida Shakiba1 , Shirin Haghighat2 , Marzieh Jamalidoust3 , Jamal Sarvari4
1- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.| Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.| Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract: (1192 Views)
Background: The exact cause chronic lymphocytic leukemia (CLL) is still unknown. Cytomegalovirus (CMV) may play a role in the development of CLL, Therefore, the aim of this study is to investigate the frequency of CMV in patients with CLL and its relationship with blood and genetic factors.
Methods: This cross-sectional study was conducted between April 2020 and October 2022 on 40 CLL patients that referred to Dr. Daneshbod Pathobiology Laboratory (Shiraz, Iran). After taking blood and separating the buffy coat, viral DNA was extracted using a commercial DNA extraction kit and the CMV burden was measured using Real-time PCR assay. Moreover, a blood cell count test was performed. The amount of lactate dehydrogenase of the serum was measured using the kit. Also, common chromosomal disorders and CD38 marker related data were extracted from the file patients. SPSS software and Student's t-test were used to result analysis.
Results: The mean age of the patients was 62.25 ± 10.49 years. Of the 40 patients, 28 were men (70%). The average number of white blood cells was 46.06±1.49* 109, which was significantly higher in women than in men (p=0.031). Real-time PCR results showed that two patients (5%) have detectable amounts of CMV virus genome. The level of lactate dehydrogenase, CD38 marker, and the number of malignant cells in male and female patients did not differ significantly (p=0.362). Moreover, chromosomal abnormalities include deletions in 11q (ATM) and 17P (TP53), were observed in 3 (7.5%) and 4 (10%) patients, respectively.
Conclusion: Our finding indicated the CMV might not involve in the pathogenesis of CLL disease. More studies are recommended for clarify this finding.
Methods: This cross-sectional study was conducted between April 2020 and October 2022 on 40 CLL patients that referred to Dr. Daneshbod Pathobiology Laboratory (Shiraz, Iran). After taking blood and separating the buffy coat, viral DNA was extracted using a commercial DNA extraction kit and the CMV burden was measured using Real-time PCR assay. Moreover, a blood cell count test was performed. The amount of lactate dehydrogenase of the serum was measured using the kit. Also, common chromosomal disorders and CD38 marker related data were extracted from the file patients. SPSS software and Student's t-test were used to result analysis.
Results: The mean age of the patients was 62.25 ± 10.49 years. Of the 40 patients, 28 were men (70%). The average number of white blood cells was 46.06±1.49* 109, which was significantly higher in women than in men (p=0.031). Real-time PCR results showed that two patients (5%) have detectable amounts of CMV virus genome. The level of lactate dehydrogenase, CD38 marker, and the number of malignant cells in male and female patients did not differ significantly (p=0.362). Moreover, chromosomal abnormalities include deletions in 11q (ATM) and 17P (TP53), were observed in 3 (7.5%) and 4 (10%) patients, respectively.
Conclusion: Our finding indicated the CMV might not involve in the pathogenesis of CLL disease. More studies are recommended for clarify this finding.
Type of Study: Brief Report |
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