Volume 56, Issue 2 (30 1998)                   Tehran Univ Med J 1998, 56(2): 4-8 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimfar M, Nuri S, Minaiy B, Barbarestani M. Study of lead effect on liver parenchymal cell of fetal rats by electron microscope. Tehran Univ Med J. 1998; 56 (2) :4-8
URL: http://tumj.tums.ac.ir/article-1-1566-en.html
Abstract:   (5393 Views)
To study the lead effect, we selected 14 females rats in which we had observed vaginal plague after mating, and divided them into a group of 8 female rats as the case group and 6 females as the control group. As drinking water the case group was given 0.13% lead acetate solution and the control group pure distilled water. Immediately after parturition the pups were killed and their livers removed. A small part of the liver was fixed in glutaraldehyde and osmium tetroxide in order to be studied with transmission electron microsope. The fixated parts were dehydrated and embed in Epon 812. using ultramicrotomoe, we prepared semithin seclions of the tissues, stained them with toludine blue, and studied them under light microscope. Further more we prepared thin sections of the tissue to be placed on grids coated with formvar. These sections were stained by uranyl acelate and lead citrate. Electron microscopic study was performed on them. Results: T.E.M. study showed the swelling and blebbing of nuclear envelop and endoplasmic reticulum and mithochondria of hepatocytes.
Full-Text [PDF 886 kb]   (1296 Downloads)    

Add your comments about this article : Your username or Email:

© 2020 All Rights Reserved | Tehran University Medical Journal TUMS Publications

Designed & Developed by : Yektaweb