Volume 68, Issue 7 (7 2010)                   Tehran Univ Med J 2010, 68(7): 377-383 | Back to browse issues page

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M D, AA M, E G. Developmental effects of Di 2 ethylhexylphthalate on ovarian follicles in Wistar rats. Tehran Univ Med J. 2010; 68 (7) :377-383
URL: http://tumj.tums.ac.ir/article-1-319-en.html
1- , mdorostghoal@yahoo.com
Abstract:   (3863 Views)

Background: In the recent years, concerns have been raised about the incidence of reproductive disorders in human populations. The present study was aimed to determine the effects of maternal exposure to Di 2 ethylhexylphthalate (DEHP) on postnatal development of ovary in Wistar rat offsprings.

Methods: Forty female Wistar rats were randomly divided in four equal experiment groups an oil vehicle group and three DEHP-treated groups that received 10, 100 and 500 mg/kg/day Di 2 ethylhexylphthalate by gavage during lactation, respectively. The ovaries of pups were removed at 60 days of postnatal development their weights recorded and fixed in Bouin's solution subsequently 6 µm serial paraffin sections were stained with haematoxylin-eosin the structural changes of ovarian follicles and corpora lutea were studied.

Results: There was no significant difference on mean body weights of offsprings among different groups. However, the mean of ovary weight was decreased significantly (p= 0.037) in 500 mg/kg/day DEHP group. Significant decreases were seen in mean number of primary follicles (p= 0.012) and mean number and diameter of secondary (p= 0.023 and p= 0.012, respectively) and antral (p= 0.025 and p= 0.018, respectively) follicles in high dose DEHP-treated group compared to sham group. Also, mean number of corpora lutea decreased significantly (p= 0.023) at 60 days of age in ovary of offspring in 500 mg/kg/day DEHP group. Moreover, significant increases were seen in number of atretic follicles in moderate (p= 0.012) and high (p= 0.036) DEHP-treated groups.

Conclusion: Present study showed that maternal exposure to Di 2 ethylhexylphthalate during lactation affects postnatal development of ovary in offspring Wistar rats and reduces their fertility and reproductive efficiency at puberty.

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