Volume 82, Issue 1 (April 2024)
Tehran Univ Med J 2024, 82(1): 71-84 |
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Shahraki S, Tavakkoli M, Khajavirad A, Moghadam Matin M, Aslzare M. Creation of the suitable natural human kidney scaffold: comparison of 2 decellularization methods. Tehran Univ Med J 2024; 82 (1) :71-84
URL: http://tumj.tums.ac.ir/article-1-13000-en.html
URL: http://tumj.tums.ac.ir/article-1-13000-en.html
Samira Shahraki1 
, Mahmoud Tavakkoli2 , Abolfazl Khajavirad3 , Maryam Moghadam Matin4 , Mohammad Aslzare * 5
, Mahmoud Tavakkoli2 , Abolfazl Khajavirad3 , Maryam Moghadam Matin4 , Mohammad Aslzare * 5
1- Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.| Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
2- Department of Urology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3- Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.| Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
5- Department of Urology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. ,Zarem@mums.ac.ir
2- Department of Urology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3- Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.| Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
5- Department of Urology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. ,
Abstract: (114 Views)
Background: A range of diseases can result in end-stage renal disease (ESRD), characterized by a gradual decline in kidney function and associated with significant morbidity and mortality. Currently, renal transplantation as the most effective treatment for managing ESRD. Tissue engineering presents a considerable opportunity to expand the available supply of donor organs for kidney transplants. The aim of this research was to develop a suitable technique for preparing decellularized kidney scaffolds from human tissues.
Methods: The present study was carried out from April 2019 to August 2019 in Mashhad University of Medical Sciences. In this study, two decellularization protocols were compared using sections of human kidney tissue. Therefore, two human kidneys which collected from Ghaem and Imam Reza hospitals were used. In the first protocol, detergents such as 1% Triton X-100 (1A) and 1% SDS (Sodium Dodecyl Sulfate) (1B) were employed, followed by the application of DNase I. The second protocol utilized 0.5% SDS (2A) and 1% SDS (2B). The effectiveness of these techniques was evaluated using hematoxylin and eosin (H&E) staining, 4',6-diamidino-2-phenylindole (DAPI), DNA quantification, and immunohistochemistry (IHC).
Methods: The present study was carried out from April 2019 to August 2019 in Mashhad University of Medical Sciences. In this study, two decellularization protocols were compared using sections of human kidney tissue. Therefore, two human kidneys which collected from Ghaem and Imam Reza hospitals were used. In the first protocol, detergents such as 1% Triton X-100 (1A) and 1% SDS (Sodium Dodecyl Sulfate) (1B) were employed, followed by the application of DNase I. The second protocol utilized 0.5% SDS (2A) and 1% SDS (2B). The effectiveness of these techniques was evaluated using hematoxylin and eosin (H&E) staining, 4',6-diamidino-2-phenylindole (DAPI), DNA quantification, and immunohistochemistry (IHC).
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Results: Based on H&E staining results, comparison of the decellularized and native human kidney tissues showed a successful elimination of cell nuclei and the ameliorate extracellular matrix preservation in triton-treated scaffolds (1A) in comparison with the SDS-treated scaffolds (1B) at all times protocols. Furthermore, DNA quantification illustrated triton X-100 in removing DNA was more effective in eliminating DNA from kidney tissues compared to other protocols in renal tissues. In addition, IHC staining demonstrated that the expression of collagen IV and laminin was preserved throughout the decellularization process with Triton X-100 on day fifth. Also, IHC staining indicated human leukocyte antigen (HLA) was completely eliminated in the cortex-medulla of human scaffolds treated with Triton X-100 within day fifth.
Conclusion: Our results demonstrated that triton X-100 outperformed SDS as a detergent for decellularizing human kidneys. Meanwhile these results indicate suitable method for decellularization of human kidneys to produce functional kidneys. |
Keywords: human kidney, Extracellular matrix, decellularization, triton x-100, sodium dodecyl sulfate.
Type of Study: Original Article |
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