Volume 55, Issue 3 And 4 (30 1997)                   Tehran Univ Med J 1997, 55(3 And 4): 7-11 | Back to browse issues page

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Shagari G, Sarrafnegad A, Behzadiannegad G. Study of endotoxin release by Salmonella thyphi by antibiotics in Chromogenic Limulus Amebocyte Lyssate Method. Tehran Univ Med J. 1997; 55 (3 and 4) :7-11
URL: http://tumj.tums.ac.ir/article-1-1643-en.html
Abstract:   (5514 Views)
It seems, rapid destruction of gram negative bacteria by antibiotics contribute to the clinical deterioration of some patients with lethal endotoxemia. In this research we evaluated LPS (lipopolysaccharide) release during antibiotic killing of salmonella typhi (Ty2-5536). The organism was incubated in the presence of Chloramphenicol, Ampicillin and Co-trimoxazole at concentrations that killed >99.9% of the organisms as determined by quantitative culture techniques. After incubation the antibiotic-bacterial cultures were centrifuged and the supernatants were filtered and collected for "in vivo" and "in vitro" studies. Injection of 1 ml/kg of filtrates in rabbits raised normal temperature of the animals by 1.2°C that it showes the presence of LPS in the filtrates. Quantitative chromogenic limulus amebocyte lyssate (L.A.L) assay was used to determine the amount of LPS in the filtrates. The amount of LPS was 86.67±2.53 Pg/ml for chloramphenicol, 113.33±8.07 Pg/ml for Ampicillin and 134.18±11.59 Pg/ml for Co-trimoxazole. According to our investigation chloramphenicol is the best antibiotic against S.typhi because it decrease the induced-pathological effects of LPS in gram negative infection
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