Inhibition of breast cancer metastasis by using miR-31-mimic in cancer stem cell rich MDA-MB231 cell line

Farokhimanesh , Samila; Forouzandeh Moghadam , Mahdi; Ebrahimi , Marzieh

Volume 73, Issue 1 (April 2015) Tehran Univ Med J 2015, 73(1): 1-10 | Back to browse issues page

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Farokhimanesh S, Forouzandeh Moghadam M, Ebrahimi M. Inhibition of breast cancer metastasis by using miR-31-mimic in cancer stem cell rich MDA-MB231 cell line. Tehran Univ Med J 2015; 73 (1) :1-10
URL: http://tumj.tums.ac.ir/article-1-6567-en.html

Inhibition of breast cancer metastasis by using miR-31-mimic in cancer stem cell rich MDA-MB231 cell line

Samila Farokhimanesh¹

, Mahdi Forouzandeh Moghadam ^*

², Marzieh Ebrahimi³

1- Department of Biotechnology, Faculty of Medical Science, Tarbiat Modares University, Teh-ran, Iran.
2- Department of Biotechnology, Faculty of Medical Science, Tarbiat Modares University, Teh-ran, Iran. , foroz@modares.ac.ir
3- Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for StemCell Biology and Technology, ACECR, Tehran, Iran.

Abstract: (7046 Views)

Background: Metastasis associated miRNA (metastamiR) opened a new field of anti-metastatic therapy which have a great potential of treatment for the most lethal aspect of cancer, metastasis. The pleiotropic nature of gene regulation exhibited by certain miRNAs that showed that miRNAs might be endowed with a capacity to function as crucial modulators of tumor metastasis. MiR-31 is a pleiotropic anti-metastatic miRNA whose expression decreased significantly in metastatic breast cancer cells. MiR-31 has multiple roles in metastasis cascade. Therefore, using the miR-31-restoration based therapy could be an efficient anti-metastatic strategy for cancer therapy. Methods: This research was performed from May 2014 to October 2015 in Tarbiat Modares University in Tehran, Iran. The double-strand oligo of mature miR-31 was cloned into pcDNA 6.2gw/EmGFP according to the manufacturer instruction. The MDA-MB231, MCF-7 breast cancer cell lines were cultured and their miRNAs have been extracted. The expression of miR-31 has been quantified by Real time-PCR be-fore transfection of construct contained miR-31 into two cell lines and in normal breast cells. Then the constructs contain miR-31 have been transfected in to two cell lines. The expression of miR-31 has been quantified after 48 hours. Scratch and invasion as-say have been carried out for assessing the level of migration and invasion. Results: The result of Real time-PCR before transfection of constructs contained miR-31 have been shown 4 fold and more than 100 fold reduction in expression of miR-31 in MCF-7 and MDA-MB231 respectively in comparison to miR-31 expression in nor-mal breast cells, but after transfection of miR-31 construct to MDA-MB231 the quan-tification of expression showed the significant increase in mir-31 expression and 20 fold reduction in invasive and 10 fold reduction in migratory characteristics of MDA-MB231 in comparison to MCF-7. Conclusion: Metastasis associated miRNA have been represented a promising candi-dates in the field of anti-metastatic therapy and miR-31 as a powerful member of this family can function very effectively in order to inhibit the metastasis and introduce the new possibility of metastasis inhibition.

Keywords: breast neoplasms, miR-31-mimic, neoplasm metastasis

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Type of Study: Original Article |